![]() Schluepmann, H., Dijken, A., Aghdasi, M., Wobbes, B., Paul, M. Tomato abiotic stress enhanced tolerance by Trehalose biosynthesis. A fluorometric assay for Trehalose in the picomole range. Plant Physiology, 144(1), 3-5.Ĭarillo, P., Feil, R., Gibon, Y., Satoh-Nagasawa, N., Jackson, D., Bläsing, O.E., Stitt, M. The role of Trehalose biosynthesis in plants. Fine tuning of Trehalose biosynthesis and hydrolysis as novel tools for the generation of abiotic stress tolerant plants. Carbon/nitrogen imbalance associated with drought-induced leaf senescence in Sorghum bicolor. Journal of Integrative Plant Biology, 50, 1223-1229.Ĭhen, D., Wang, S., Xiong, B., Cao, B. Trehalose biosynthesis in response to abiotic stresses. ![]() Compatible solute engineering in plants for abiotic stress tolerance - role of glycine betaine. Organic compatible solutes of halotolerant and halophilic microorganisms. Journal of Experimental Botany, 53(336), 13-25. Molecular and physiological approaches to field maize improvement for drought tolerance. Effects of drought stress and water recovery on physiological responses and gene expression in field maize seedlings. Research of Maize Breeding for Drought Tolerance. Keywords: cDNA probe stay-green phenotype S 2 maize families image processing relative signal intensity Furthermore, we found that 4 out of 34 S 2 maize families may have potential for further use in our breeding program. It is noteworthy that the study of TPS gene expression in mature maize under stress in this study showed results that contrasted with previous reports on seedlings in many plant species. Our study showed that maize with high TPS gene expression tended to be less tolerant to water stress. Furthermore, a moderate negative relationship between relative signal intensity at 66 DAP (RI 66) and Smith index based on multi-phenotypic traits was found to be statistically significant. However, dot blotting at 6 days after stress (relative intensity at 66 DAP) was effective to differentiate maize families. The results showed that the level of TPS gene expression was highest at 4 days after stress (relative intensity at 64 DAP). Leave samples were collected at 6 different days after planting (DAP) for a dot blot assay. To do so, 34 S 2 maize families were grown and subjected to water stress condition. The aim of this research was to screen the levels of TPS gene expression in maize breeding materials under water stress via dot-blot hybridization using cDNA probe. Trehalose-6-phosphate synthase (TPS) is an important enzyme involved in trehalose biosynthesis which has been found to increase plant tolerance to abiotic stresses. One way to alleviate yield losses is to develop drought tolerant maize varieties for farmers. Maize yields have been inadequate for the demand due to drought events. Field maize is an important economic crop grown around the world and it has been mainly used in the animal feed industry.
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